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Succeed Equine Fecal Blood Test - 10 Pack : VetDepot.com

Succeed Equine Fecal Blood Test - 10 Pack

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Succeed Equine Fecal Blood Test - 10 Pack is used to test if your horse has a problem with its digestive tract. Because ulcers are a common in horses, Succeed Blood Test can help let you know if your horse is suffering from one. This test is the only reliable way to detect occult fecal blood from anywhere in your horse's GI tract. Please Note: This product requires overnight expedited shipping and ships Mon. through Thurs. During summer, purchasing 1 or 2 extra ice packs is advised.
Description
SUCCEED® EQUINE FECAL BLOOD TEST™

Freedom Health

Rapid dual antibody test detects albumin and hemoglobin in equine feces.

Aids diagnosis of GI tract conditions in horses.

A Breakthrough in Equine GI Health Diagnostics.

The SUCCEED® Equine Fecal Blood Test™ provides a rapid, convenient method for detecting occult albumin and hemoglobin in equine feces, which may be indicative of digestive tract lesions or other GI tract conditions, and which can help distinguish foregut from hindgut conditions.

READ ALL INSTRUCTIONS BEFORE USE.

Use Instructions and Technical Information

Contents

Contains one complete SUCCEED® equine fecal occult blood test, including:

- 1 clear plastic container

- 1 dual test cassette - contains both an albumin test (Test A) and a hemoglobin test (Test H)

- 1 disposable sample pipette

- 1 polyethylene glove

- 1 instruction sheet

- 1 desiccant pouch (for moisture)

Intended Use

The SUCCEED® Equine Fecal Blood Test™ (FBT) is a qualitative lateral-flow rapid test that detects horse hemoglobin and albumin in equine fecal specimens. The SUCCEED FBT is for veterinary use only, for use as a diagnostic aid in assessing GI tract conditions in their equine patients.

Test Mechanism

The SUCCEED FBT utilizes proprietary antibodies to equine albumin and hemoglobin, and has been calibrated to detect levels exceeding baseline bleeding, consistent with a disease condition. Test A detects albumin in feces, which indicates injury at points caudal to the common bile duct in the duodenum (or, generally in the hindgut). Test H detects hemoglobin in feces, which indicates injury anywhere along the GI tract of the horse. Taken together, the two parts of the test may provide a means of distinguishing foregut and hindgut conditions in horses.

Precautions

- For veterinary use only.

- For in vitro diagnostic use.

- Do not use after expiration date, which is printed on the back of the test cassette.

- Keep specimen clear of any potential contaminants. Use only the provided plastic container to collect and mix the fecal sample.

- Keep test cassette in original, closed container until use. Exposure to moisture will affect antibodies.

- Product may be safely shipped throughout the year, and safely used in temperatures between 5° C and 30° C (40° F to 85° F).

- Store in original packaging in temperatures below 27° C (80° F). Keep out of direct sunlight or heat sources.

Patient Considerations

1. Check horse for signs of external bleeding where blood may be introduced to the digestive tract, particularly around the mouth.

2. Avoid testing horses within 48 hours of racing, especially those prone to bleeding (i.e., exercise induced pulmonary hemorrhage) where horse may swallow blood.

3. Avoid testing horses within 48 hours of having their teeth floated.

4. Avoid testing mares during ovulation or immediately post-partum, where bleeding may be present.

Instructions

1. Remove all items from the plastic container and set aside. Do not discard container lid. Discard desiccant pouch. (Desiccant pouch is not edible. Discard appropriately.)

2. Using provided glove, carefully collect fecal material from a single, fresh, complete bowel movement of subject horse. (Feces should be as fresh as possible, never more than 4 hours old.) Collect about a pinch from multiple locations in a complete fecal specimen. Drop each pinch-sized sample into the plastic container up to the sample fill line (lower line) on the label. Allow fecal matter to sit loosely in the container - do not pack or compress.

3. Add clean tap water to the fecal matter in the container. Fill to the water fill line (top line). Do not use water from dirty containers or from sources that may be contaminated with equine blood.

4. Replace cap on container, and shake vigorously to mix fecal matter and water. Set container on a flat surface, and remove lid.

5. Use provided disposable sample pipette to collect some of the solution from the container. Collect liquid only, avoiding particles of fecal matter.

6. Using provided sample pipette, apply 2 drops of solution to EACH of the two sample wells on the test cassette. Watch for liquid to wick up the membrane in the test window. Apply one or two additional drops if needed. Be careful not to flood the test membrane.

7. Read results. A positive result may appear within 5 minutes after solution has clearly wicked up test membrane, but may take longer. DO NOT read results after 15 minutes.

Reading the Tests

Observe results after applying the solution to each of the two sample wells on the cassette.

1. If a Control line does not appear within 15 minutes (at the CTL point on the cassette) that test result is invalid. The test must then be repeated with a new SUCCEED Equine Fecal Blood Test.

2. If only a Control line appears on either strip, that test is NEGATIVE. NOTE: only a complete line is a meaningful result.

If a solid line appears only at the CTL point (Control line) on either Test A or Test H, this is a NEGATIVE result.

3. If both the Control and Test lines appear on either window (Test A or H), this indicates a POSITIVE test result. Even if the test line is faint, the test is POSITIVE.

If a complete, solid line appears at the TST point (Test line) on either or both Test A and Test H, that is a POSITIVE test result. Test A detects equine albumin. Test H detects equine hemoglobin.

Note - even if the test line is faint, the test is POSITIVE.

4. Do not read test results after 15 minutes.

If you have questions about reading the SUCCEED Equine Fecal Blood Test, please call: 800-281-6727 from 8AM - 5PM ET Monday through Friday.

Interpreting the Results

The following chart summarizes the meaning of a positive and/or negative test results across SUCCEED FBT tests A and H.

 

Test A

Albumin

Negative

Positive

Test H

Hemoglobin

Negative

No GI tract issue detected.

GI tract issue detected in hindgut.

Positive

GI tract issue detected in foregut.

GI tract issue detected in hindgut. Foregut issue also possible.*

* Note that Test A uses an antibody for albumin, which typically breaks down in digestion, particularly when exposed to digestive enzymes in the proximal portion of the small intestine. Thus a positive Test A will indicate bleeding from a source caudal to the duodenum (e.g. hindgut). Also, because albumin may be present at the site of low-grade injury absent whole blood, you may get a positive Test A in response to a low-grade lesion in the hindgut, such as may occur with heavy parasitism or disseminated grade 1 colonic ulcers.

Test H uses an antibody for hemoglobin, which is more resistant to enzymatic activity. Thus a positive Test H, with negative Test A, will indicate foregut blood loss. You will not obtain a positive result from either Test A or H when a horse has a foregut lesion equivalent to a grade 1 gastric ulcer, where whole blood loss does not occur.

Limitations of the Test

The SUCCEED FBT is a valuable tool for the qualitative detection and

location of gastrointestinal bleeding. It is not a quantitative test.

Equine albumin and hemoglobin in feces may arise from a variety

of conditions, including but not limited to:

- Bleeding ulcers (gastric or colonic)

- Excessive parasitic activity

- Protein-losing enteropathy (PLE)

- Lesions caused by hard particles of feed or other ingested material

- Petechiation, hyperemias or lypomas

Equine GI lesions - including petechiation and parasite pits - may bleed lightly or intermittently, so a test result may be negative even when disease is present. And because blood may be non-uniformly distributed in a fecal sample, positive readings may occasionally be obtained from healthy horses. As with any fecal blood test, SUCCEED FBT is not a conclusive diagnostic for gastrointestinal bleeding or pathology; it should be employed only as an aid to diagnosis.

Additionally, a positive test result (especially Test H), can arise from extraneous sources, such as:

- Dentistry or exercise-induced pulmonary hemorrhage

- Mare ovulation

- Post-surgery bleeding

As with any diagnostic, a definitive diagnosis should not be based on the result of a single test. The test is designed to be complementary to blood tests and physical examination. For practitioners experiencing a positive Test A without a positive Test H, one of the differential diagnoses should include a protein-losing enteropathy, especially in the presence of hypoproteinemia/hypoalbuminemia on a CBC/chem profile.

Failure to carefully follow the instructions in this sheet may lead to incorrect interpretation.

Applications

Because symptoms are an unreliable indicator of GI health, practitioners should test horses periodically. Given the ease and affordability of the FBT, testing all horses on a regular schedule is a practical option for early detection. The SUCCEED FBT may also be an important part of a wellness program.

Consistent testing is especially important for performance horses, or wherever the care, feeding and general husbandry are less than ideal for digestive health, including intermittent feeding, high-grain diets, stall confinement, etc. Because it is possible that GI health may be a root cause of many performance or behavioral problems, horses with such issues may be prime candidates for testing.

The SUCCEED FBT may also be a valuable resource to aid in monitoring blood loss following colic surgery, or as part of a pre-purchase exam.

Performance Characteristics

Multiple tests, both in vitro and in vivo, were completed to assess the performance of the SUCCEED FBT, as follows.

Analytical Sensitivity

The antibodies used in the two parts of the SUCCEED FBT - Test A and Test H - were evaluated by introducing equine blood at varying concentrations into a solution of water and rabbit fecal matter. The FBT antibodies have the following detection limits at a 95% confidence level:

Figure 1 - Minimum detection levels of FBT antibodies

 

Test A
Antibody

Test H
Antibody

Detection Limit

8 ppm

7 ppm

Statistical Accuracy

The following indicates the diagnostic sensitivity and specificity of the SUCCEED FBT, comparing test results on fecal samples with observed GI tract injury in equine subjects post-necropsy:

Figure 2 - Analysis of SUCCEED® FBT Test A and Test H as indicators of ulceration

N =86

Test A (Colonic)

Test H (Full GI Tract)

Sensitivity

83%

75%

Specificity

73%

87%

Predictive Value Pos.

95%

96%

P-Value

3%

2%

The SUCCEED FBT has been calibrated to only reflect true pathologies and to ignore subclinical or baseline bleeding.

Incidence of GI Tract Injury

Based on necroscopic analysis of equine subjects, the following incidence levels of various disease states were observed in two studies:

Figure 3 - Incidence of GI tract conditions

Condition

Study 1 - N = 188
Incidence

Study 2 - N = 111
Incidence

Gastric Ulceration

47.9%

43.2%

Colonic Ulceration

74.5%

84.7%

Hyperemia

13.3%

NR

Lypoma

6.9%

NR

Petechiation

3.2%

NR

(NR = Not recorded.)

Analytical Specificity

The extent to which the SUCCEED® FBT cross-reacts with the blood of species other than horses was assessed. This was done by measuring minimum detectable concentration limits when introducing blood from various species into the samples.

Figure 4 - Minimum Detectable Limit of FBT Antibodies with Non-Equine Species Blood

Sample

Test A

Test H

Rabbit

ND

ND

Goat

ND

ND

Sheep

ND

ND

Cow

ND

ND

Pig

ND

ND

Camelid

ND

ND

As shown in Figure 4, Tests A and H are both highly specific to equine blood.

(ND = No detection at any concentration.)

SUCCEED® and Digestive Conditioning Program® are registered trademarks, and Equine Fecal Blood Test™ and SmartSignal™ are trademarks of Freedom Health, LLC. ©2009.

All Rights Reserved. PATENT PENDING.

Contact Information:

Freedom Health, LLC, 65 Aurora Industrial Parkway, Aurora, OH 44202

Toll-free service line: 877-734-6558 24 hours a day

Also available from Freedom Health:

SUCCEED® Digestive Conditioning Program® in oral paste or top-dress granules.

I501 Rev F 07/09, L501 Rev C 10/08

Presentation: 10 pack.

NAC No.: 13330020

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This information is for general reference only and should not be used for the diagnosis or treatment of any condition of your pet. Because is intended as a general reference, this information may not include all possible uses, precautions, directions, reactions (including allergic), drug interactions, or withdrawal times. Always consult your local veterinarian and have your pet examined for any advice concerning the diagnosis and treatment of your pet, including which products and doses are most appropriate. Any trademarks are the property of their respective owners. VetDepot is not a pharmacy. All prescription products are dispensed by our Pharmacy Partner.


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